A remarkable example of this approach regards the development of nano-enhanced disposable MALDI mass-spectrometry plates for Proteomics, Microbiology and advanced Histology.
Here, a nanostructured oxide film having suitable dot-patterned shape is deposited, with a proprietary technology, onto a conductive substrate. Nanoscale porosity is exploited to capture samples and allow processing directly on-plate, into the nanostructured dots, which act as a “solid-state microwell”. Secondary cleaning by water wash (e.g. for salt removal) and Trypsin digestion, in the case of protein samples; Formic Acid membrane lysis in the case of some bacteria/yeast samples, are just few examples of possible on-plate sample processing. An effective hydrophobic barrier at dot border prevents samples, washing solutions and reagents to spread outside the dot area during on-plate processing, as tutorial videos show.
Credits M. Lalowski